4-NITROBENZOIC ACID (PNB), 25 g, powder, vial
Valid Article
OC subscriptions: included in supply or field order tools (e.g. UniField)
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SC subscriptions: included in supply or field tools
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OC validations: approved for procurement and use by an OC for international or local orders specifying context and activity in Medical Standard Lists (MSL)
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4-NITROBENZOIC ACID (PNB)
Reserved for TB culture programmes.
Definition
This reagent is added to TLA or LJ medium for primary identification of Mycobacterium tuberculosis complex.
Synonym
p-Nitrobenzoic acid, PNB, p-nitrobenzenecarboxlyic acid; 1-carboxy-4-nitrobenzene
Specifications
Technical specifications
- CAS n°: 62-23-7
- Formula: C7H5NO4
- Purity: 98%
- Quality: pure
- White to yellow powder or crystals or crystalline powder
Packaging & Labelling
25 g in bottle
Transport Dangerous Goods
Transport not regulated
Instructions for use
This reagent is used to identify M. tuberculosis complex (MTBc). The principle is that MTBc is inhibited by ρ-Nitrobenzoic acid (PNB), whereas non-tuberculous mycobacteria (NTM) are resistant. Primary identification is done by the comparison of growth among the two media.
PNB is added to Middlebrook 7H11 or Lowenstein-Jensen during medium preparation. Follow Standard Operational Procedures for stock solution preparation and plates/tubes preparation.
Storage
- At room temperature
- Keep tightly closed
- 4- nitrobenzoic acid solution should be used immediately, or stored in the refrigerator.
Waste management
Substance with hazardous characteristics. Please contact your watsan referent for advice on proper disposal.
Detailed hazard and precautionary information can be found in the safety data sheet (SDS).
Classification EC Regulation N° 1272/2008
Variable between manufacturers. Check the SDS from your product for exact values and exact lists of H and P statements.
As example: Acros Organics
- Acute toxicity, Oral: Category 4, H302
- Serious eye damage: Category 1, H319
Signal Word
Danger
| H302 | Harmful if swallowed |
| H315 | Causes skin irritation |
| H319 | Causes serious eye irritation |
| H335 | May cause respiratory irritation |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection |
| P301 + P312 | IF SWALLOWED: call a POISON CENTER or doctor/physician if you feel |
| P305 + P351 + P338 | IF IN EYES: rinse cautiously with water for several minutes. If the victim wears contact lenses, remove them if possible. Keep rinsing |
MSF requirements
ρ-Nitrobenzoic acid (PNB) is used in TLA and LJ cultures for the primary identification of Mycobacterium tuberculosis (MTBc) against non-tuberculous mycobacteria (NMT).